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KMID : 0603520030080020124
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Journal of Korean Association of Cancer Prevention
2003 Volume.8 No. 2 p.124 ~ p.132
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The Anti-carcinogenic Effect of Korean Cabbage Extracts on the Inhibition of Gap Juctional Intercellular Communication by Hydrogen Peroxide and 12-O-Tetradecanoylphorbol-13-acetate
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Hwang Jae-Woong
Jung Ji-Won
Ahn Nam-Shik
Yang Se-Ran
Park Joon-Suk
Jo Eun-Hye
Yoon Byong-Su
Kim Sung-Hoon
Lee Yong-Soon
Kang Kyung-Sun
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Abstract
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Modulation of gap junctional intercellular communication (GJIC) is a known cellular event associated with tumor promotion. We examined the anti-carcinogenic properties of the Korean cabbage extracts (DF-01, DF-02, DF-03, Seoul, GM-A, GM-B) on the inhibition of GJIC by hydrogen peroxide (H2O2) or phorbol ester (12-O-Tetradecanoylphorbol-13-acetate; TPA) in WB-F344 rat liver cell (WB cells). The cells were pre-incubated with Korean cabbage extracts within non-cytotoxic concentration for 24 h followed by co-treatment with extracts and H2O2 (750μM) or TPA (10 ng/ml) for 1 h. DF-01, DF-02, and DF-03 prevented the inhibition of GJIC by H2O2. Inhibition by TPA of GJIC was not prevented by any extracts. To find out the action mechanism of Korean cabbage extracts, western blot analysis was performed to show whether, DF-01 and DF-03 block connexin 43 hyperphosphorylation or inhibit extracellular signal-regulated protein kinases (ERK) 1/2 activation by H2O2 in WB cells. Korean cabbage extracts such as DF-01 and DF-03 prevented H2O2 induced GJIC inhibition through inactivation of ERK1/2 mitogen-activated protein (MAP) kinases. Therefore, it is suggested that the Korean cabbage extracts might have the potential chemopreventive effects by the inhibition of GJIC.
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KEYWORD
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Korean cabbage extracts, Gap junctional intercellular communication(GJIC), WB-F344 rat liver cells (WB cells), Connexin 43, Extracellular signal-regulated protein kinase (ERK)1/2
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